A single amino acid substitution in a common african allele of the CD4 molecule ablates binding of the monoclonal antibody, OKT4
Identifieur interne : 004954 ( Main/Exploration ); précédent : 004953; suivant : 004955A single amino acid substitution in a common african allele of the CD4 molecule ablates binding of the monoclonal antibody, OKT4
Auteurs : Seth Lederman [États-Unis] ; Julie A. Demartino [États-Unis] ; Bruce L. Daugherty [États-Unis] ; Ivan Foeldvari [États-Unis] ; Michael J. Yellin [États-Unis] ; Aileen M. Cleary [États-Unis] ; Noah Berkowitz [États-Unis] ; Israel Lowy [États-Unis] ; Ned S. Braunstein [États-Unis] ; George E. Mark [États-Unis] ; Leonard Chess [États-Unis]Source :
- Molecular Immunology [ 0161-5890 ] ; 1991.
English descriptors
- Teeft :
- Acad, Acid substitution, Aids retrovirus, Allele, American type culture collection, Amino, Amino acid, Amino acid residue, Amino acid sequence, Amino acid substitution, Amino acids, Amplimer sequence, Antibody responses, Aozasa, Arginine, Arginine tryptophan substitution, Autoimmune disease, Axel, Binding site, Biochemical analysis, Cdna, Cdna encoding, Cell activation, Cell line, Cell receptor, Cell surface, Clone, Coding region, Coding sequence, Columbia university, Differentiation antigen, Direct sequencing, Ecor1 fragment, Encoding, Envelope glycoprotein, Epitope, Ethidium bromide, Full length cdna, Full length molecule, Functional consequences, Functional defect, Gene encoding, Genetic basis, High prevalence, Human cytotoxic, Human immunodeficiency virus, Human inducer, Human sequence, Immun, Large deletion, Lymphoblastoid cells, Lymphocyte, Mabs, Maddon, Magnetic beads, Mcdougal, Molecular basis, Molecule, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Mutation, Natn, Nucleic acids, Nucleotide, Nucleotide sequence, Okt4, Okt4 allele, Okt4 alleles, Okt4 phenotype, Okt4a, Phenotype, Physical association, Plasmid, Polymerase chain reaction, Polymorphism, Present study, Primer, Primer pairs, Proc, Protein beads, Receptor, Reinherz, Restriction sites, Seth ledermanet, Signal segment, Signal sequence, Stohl, Substitution, Syncytium formation, Systemic lupus erythematosus, Terminus, Tripartite leader.
Abstract
Abstract: The CD4 molecule is a relatively non-polymorphic 55 kDa glycoprotein expressed on a subset of T lymphocytes. A common African allele of CD4 has been identified by non-reactivity with the monoclonal antibody, OKT4. The genetic basis for the OKT4− polymorphism of CD4 is unknown. In the present paper, the structure of the CD4 molecule from an homozygous CD4OKT4− individual was characterized at the molecular level. The size of the CD4OKT4− protein and mRNA were indistinguishable from those of the OKT4+ allele. The polymerase chain reaction (PCR) was used to map the structure of CD4OKT4− cDNAs by amplifying overlapping DNA segments and to obtain partial nucleotide sequence after asymmetric amplification. PCR was then used to clone CD4OKT4− cDNAs spanning the coding region of the entire, mature CD4 protein by amplification of two overlapping segments followed by PCR recombination. The nucleotide sequence of CD4OKT4− cDNA clones revealed a G → A transition at bp 867 encoding an arginine → tryptophan substitution at amino acid 240 relative to CD4OKT4+. Expression of a CD4OKT4−cDNA containing only this transition, confirmed that the arginine → tryptophan substitution at amino acid 240 ablates the binding of the mAb OKT4. A positively charged amino acid residue at this position is found in chimpanzee, rhesus macaque, mouse and rat CD4 suggesting that this mutation may confer unique functional properties to the CD4OKT4− protein.
Url:
DOI: 10.1016/0161-5890(91)90003-3
Affiliations:
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Mark</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cellular and Molecular Biology, Merck, Sharp & Dohme Research Laboratories, Rahway, NJ 07065</wicri:regionArea><wicri:noRegion>NJ 07065</wicri:noRegion></affiliation></author><author><name sortKey="Chess, Leonard" sort="Chess, Leonard" uniqKey="Chess L" first="Leonard" last="Chess">Leonard Chess</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Columbia University, New York, NY 10032</wicri:regionArea><orgName type="university">Université Columbia</orgName><placeName><settlement type="city">New York</settlement><region type="state">État de New York</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Immunology</title><title level="j" type="abbrev">MIMM</title><idno type="ISSN">0161-5890</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">28</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1171">1171</biblScope><biblScope unit="page" to="1181">1181</biblScope></imprint><idno type="ISSN">0161-5890</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0161-5890</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Acid substitution</term><term>Aids retrovirus</term><term>Allele</term><term>American type culture collection</term><term>Amino</term><term>Amino acid</term><term>Amino acid residue</term><term>Amino acid sequence</term><term>Amino acid substitution</term><term>Amino acids</term><term>Amplimer sequence</term><term>Antibody responses</term><term>Aozasa</term><term>Arginine</term><term>Arginine tryptophan substitution</term><term>Autoimmune disease</term><term>Axel</term><term>Binding site</term><term>Biochemical analysis</term><term>Cdna</term><term>Cdna encoding</term><term>Cell activation</term><term>Cell line</term><term>Cell receptor</term><term>Cell surface</term><term>Clone</term><term>Coding region</term><term>Coding sequence</term><term>Columbia university</term><term>Differentiation antigen</term><term>Direct sequencing</term><term>Ecor1 fragment</term><term>Encoding</term><term>Envelope glycoprotein</term><term>Epitope</term><term>Ethidium bromide</term><term>Full length cdna</term><term>Full length molecule</term><term>Functional consequences</term><term>Functional defect</term><term>Gene encoding</term><term>Genetic basis</term><term>High prevalence</term><term>Human cytotoxic</term><term>Human immunodeficiency virus</term><term>Human inducer</term><term>Human sequence</term><term>Immun</term><term>Large deletion</term><term>Lymphoblastoid cells</term><term>Lymphocyte</term><term>Mabs</term><term>Maddon</term><term>Magnetic beads</term><term>Mcdougal</term><term>Molecular basis</term><term>Molecule</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Mutation</term><term>Natn</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Okt4</term><term>Okt4 allele</term><term>Okt4 alleles</term><term>Okt4 phenotype</term><term>Okt4a</term><term>Phenotype</term><term>Physical association</term><term>Plasmid</term><term>Polymerase chain reaction</term><term>Polymorphism</term><term>Present study</term><term>Primer</term><term>Primer pairs</term><term>Proc</term><term>Protein beads</term><term>Receptor</term><term>Reinherz</term><term>Restriction sites</term><term>Seth ledermanet</term><term>Signal segment</term><term>Signal sequence</term><term>Stohl</term><term>Substitution</term><term>Syncytium formation</term><term>Systemic lupus erythematosus</term><term>Terminus</term><term>Tripartite leader</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The CD4 molecule is a relatively non-polymorphic 55 kDa glycoprotein expressed on a subset of T lymphocytes. A common African allele of CD4 has been identified by non-reactivity with the monoclonal antibody, OKT4. The genetic basis for the OKT4− polymorphism of CD4 is unknown. In the present paper, the structure of the CD4 molecule from an homozygous CD4OKT4− individual was characterized at the molecular level. The size of the CD4OKT4− protein and mRNA were indistinguishable from those of the OKT4+ allele. The polymerase chain reaction (PCR) was used to map the structure of CD4OKT4− cDNAs by amplifying overlapping DNA segments and to obtain partial nucleotide sequence after asymmetric amplification. PCR was then used to clone CD4OKT4− cDNAs spanning the coding region of the entire, mature CD4 protein by amplification of two overlapping segments followed by PCR recombination. The nucleotide sequence of CD4OKT4− cDNA clones revealed a G → A transition at bp 867 encoding an arginine → tryptophan substitution at amino acid 240 relative to CD4OKT4+. Expression of a CD4OKT4−cDNA containing only this transition, confirmed that the arginine → tryptophan substitution at amino acid 240 ablates the binding of the mAb OKT4. A positively charged amino acid residue at this position is found in chimpanzee, rhesus macaque, mouse and rat CD4 suggesting that this mutation may confer unique functional properties to the CD4OKT4− protein.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>New York</li></settlement><orgName><li>Université Columbia</li></orgName></list><tree><country name="États-Unis"><region name="État de New York"><name sortKey="Lederman, Seth" sort="Lederman, Seth" uniqKey="Lederman S" first="Seth" last="Lederman">Seth Lederman</name></region><name sortKey="Berkowitz, Noah" sort="Berkowitz, Noah" uniqKey="Berkowitz N" first="Noah" last="Berkowitz">Noah Berkowitz</name><name sortKey="Braunstein, Ned S" sort="Braunstein, Ned S" uniqKey="Braunstein N" first="Ned S." last="Braunstein">Ned S. Braunstein</name><name sortKey="Chess, Leonard" sort="Chess, Leonard" uniqKey="Chess L" first="Leonard" last="Chess">Leonard Chess</name><name sortKey="Cleary, Aileen M" sort="Cleary, Aileen M" uniqKey="Cleary A" first="Aileen M." last="Cleary">Aileen M. Cleary</name><name sortKey="Daugherty, Bruce L" sort="Daugherty, Bruce L" uniqKey="Daugherty B" first="Bruce L." last="Daugherty">Bruce L. Daugherty</name><name sortKey="Demartino, Julie A" sort="Demartino, Julie A" uniqKey="Demartino J" first="Julie A." last="Demartino">Julie A. Demartino</name><name sortKey="Foeldvari, Ivan" sort="Foeldvari, Ivan" uniqKey="Foeldvari I" first="Ivan" last="Foeldvari">Ivan Foeldvari</name><name sortKey="Lowy, Israel" sort="Lowy, Israel" uniqKey="Lowy I" first="Israel" last="Lowy">Israel Lowy</name><name sortKey="Mark, George E" sort="Mark, George E" uniqKey="Mark G" first="George E." last="Mark">George E. Mark</name><name sortKey="Yellin, Michael J" sort="Yellin, Michael J" uniqKey="Yellin M" first="Michael J." last="Yellin">Michael J. Yellin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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